Composite

Part:BBa_K3002102:Design

Designed by: Dorothée Klein   Group: iGEM19_TU_Kaiserslautern   (2019-10-16)


Level 1 - Spectinomycin Resistance Gene for Chlamydomonas reinhardtii (Phytobrick)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1401
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1694
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon-optimized for Chlamydomonas reinhardtii. Additionally, it is compatible with Phytobricks /MoClo parts.


Source

This part is a combination of the parts BBa_K3002001 (PSAD-promoter), BBa_K3002002 (PSAD-Terminator), BBa_K3002000 (CDS of spectinomycin resistance), BBa_K3002303 (connector B2-B3) and BBa_K3002305 (connector B5-B6)

References